In Vitro Germination of Nicotiana glauca: Unlocking Its Biotechnological Potential

Authors

DOI:

https://doi.org/10.46551/ruc.v27n2a5

Keywords:

Temperature, Sorbitol, Wild tobacco, Storage, Anabasine

Abstract

Nicotiana glauca Graham (Solanaceae) produces anabasine with biotechnological applications. The propagation and utilization of N. glauca are hindered by seed dormancy and sensitivity to storage conditions. To address this challenge, this study investigated the effects of storage temperature and osmotic regulation with sorbitol on in vitro seed germination and plant establishment. Objectives: The aim was to optimize temperature storage protocols and in vitro culture conditions to improve germination rates and plant growth. Method: Seeds were stored at various temperatures (room temperature, 6°C, and -4°C) and germinated with different concentrations of sorbitol. Germination rates, plant height, and biomass were assessed. Results showed that low-temperature storage at 6°C, combined with sorbitol treatment, significantly enhanced seed viability and germination rates. This suggests that sorbitol can play a role in mitigating osmotic stress. In contrast, storage at -4°C led to decreased viability, highlighting the importance of temperature control. Conclusions: This study provides valuable insights into the factors affecting N. glauca storage seed and germination. The findings contribute to the development of efficient propagation protocols and the potential for biotechnological applications of this species. 

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Author Biographies

Thais Teixeira Valério Caetano, Universidade Federal de Lavras

Graduada em Bioquímica pela Universidade Federal de São João Del-Rei em 2018. Atualmente, discente do curso de mestrado em fisiologia vegetal pela Universidade Federal de Lavras. Atua no Laboratorio Central de Biologia Molecular, sendo orientada pelo professor Luciano Paiva. Tem experiência na área de Bioquímica, com ênfase em Biotecnologia Vegetal

 

Msc, Universidade Federal de Lavras

Possui formação em Técnico em Informática no Ensino Médio Integrado pelo Centro Federal de Educação Tecnológica de Minas Gerais (CEFET-MG - 2013), graduação em Bioquímica pela Universidade Federal de São João del Rei (UFSJ - 2018), onde fez parte do Laboratório de Biotecnologia Vegetal. Durante esse período, realizou pesquisas em cultura de tecidos vegetais, estudando a propagação in vitro de plantas dos gêneros Cannabis, Hypericum e Nicotiana. É mestre em Agronomia/Fisiologia Vegetal (2021) pela Universidade Federal de Lavras (UFLA) e atualmente cursa o doutorado em Fisiologia Vegetal, também na UFLA. Atua em linhas de pesquisa relacionadas a estresses abióticos na germinação e estabelecimento de plantas, crescimento e desenvolvimento vegetal, bioquímica e metabolismo primário.

Msc, Universidade Federal de Lavras

Mestranda no programa de Pós-Graduação em Botânica Aplicada pela Universidade Federal de Lavras (UFLA), com linha de pesquisa focada em plantas psicoativas com ênfase em biotecnologia vegetal, contemplada com bolsa CAPES. Formada em bacharel em Biotecnologia (2018-2023) pela Universidade Federal de São João del-Rei, unidade de São João del-Rei.Foi bolsista de iniciação científica (PIBITI/CNPq) sobre o tema: Potencial anticitotóxico e antigenotóxica nanopartículas com extrato de Ruta graveolens L.

Msc, Universidade Federal de Lavras

Possui graduação em Farmácia pela Universidade Federal de Minas Gerais (1983), mestrado em Química pela Universidade Federal de Minas Gerais (1989) e doutorado em Química pela Universidade Federal de Minas Gerais (1999) e, Pos-Doutorado em Química Orgânica, na Universidad de Cadiz, Cadiz, Espanha. Atualmente é professor TITULAR do CAMPUS CENTRO-OESTE DONA LINDU, UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL REI. Tem experiência na área de Farmácia, com ênfase em Farmacognosia, atuando principalmente nos seguintes temas: Plantas Medicinais Nativas do Cerrado, avaliação da qualidade de plantas medicinais comercializadas nos centros urbanos, Fitoquimica (ENFASE EM FARMACOGNOSIA), Estudo Fitoquimico de Annonaceae, Estudo Fitoquimico e Avaliaçao alelopatica visando a busca de substancias potencialmente herbicidas naturais (Química de Produtos Naturais). Professor orientou no curso de Mestrado em Química, UFMS (curso CAPES nivel 4 entre 2000-2007), no Curso de PG em Ciências da Saúde convênio interinstitucional UNB/UFMS/UFG(2005-2010). Orientou no Programa de Doutorado do Curso de Pós-Graduação em Saude e desenvolvimento na região centro-oeste (UFMS, curso CAPES nivel 4) e orientador de mestrado e DOUTORADO no Programa de Pos-Graduação em Ciencias da Saude CCO-Dona Lindu/UFSJ (CAPES nível 4) e colanorador no Programa de Pos graduação em Ciências Farmaceutica da UFSJ (nivel 3). BOLSISTA PRODUTIVIDADE no periodo de 2011-2019. PROFESSOR APOSENTADO

Vanessa Cristina Stein, Universidade Federal de Lavras

Possui graduação em Ciências Biológicas pela Universidade Federal de Pelotas (2005), Mestrado em Agronomia/Fisiologia Vegetal pela Universidade Lavras(2006), Doutorado em Agronomia/ Fisiologia Vegetal pela Universidade Federal de Lavras, com período sanduíche na Universidade de Wageningen Capes\Holanda (2009) e Pós-doutorado no Laboratório Central de Biologia Molecular da Universidade Federal de Lavras (2019/2010). Atualmente é Professa na Universidade Federal de Lavras, coordenadora adjunta do Curso de Graduação em Ciências Biológicas - Licenciatura e do Programa de Pós-graduação em Botânica Aplicada. Sua linha de pesquisa que concentra-se na aplicação de técnicas biotecnológicas para otimizar a produção de metabolitos secundários em plantas psicoativas, com ênfase nos canabinoides de Cannabis sativa (conforme autorização dos órgãos competentes) visando principalmente, alternativas para o neuropatias. Coordena o Centro Biotecnológico de Plantas Psicoativo da Ufla em convênio com o INCT-MT0 USP.

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Published

2025-08-18

How to Cite

TEIXEIRA VALÉRIO CAETANO, Thais; MOREIRA BERNARDES, Mateus; DOS REIS RONZANI, Lilian; MÁXIMO DE SIQUEIRA, João; STEIN, Vanessa Cristina. In Vitro Germination of Nicotiana glauca: Unlocking Its Biotechnological Potential. Revista Unimontes Científica, [S. l.], v. 27, n. 2, 2025. DOI: 10.46551/ruc.v27n2a5. Disponível em: https://www.periodicos.unimontes.br/index.php/unicientifica/article/view/8590. Acesso em: 15 jan. 2026.

Issue

Vol. 27 No. 2 (2025)

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Artigos Originais